Translational diffusion coefficient and molecular weight of the acetylcholine receptor from torpedo marmorata
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چکیده
منابع مشابه
Ultraviolet light-induced labeling by noncompetitive blockers of the acetylcholine receptor from Torpedo marmorata.
Reversible ligands were attached covalently to membrane-bound acetylcholine receptor from Torpedo marmorata by a method which is generally applicable and does not require the synthesis of specially designed molecules. UV irradiation of the receptor in the presence of [3H]trimethisoquin, [3H]phencyclidine, or [3H]perhydrohistrionicotoxin resulted in the labeling of the binding site(s) for these ...
متن کاملDevelopment of transient myasthenia-like symptoms in chickens injected with acetylcholine receptor from Torpedo marmorata [proceedings].
Tyrosine hydroxylase activity was determined in eight brain regions: cerebral cortex, tuberculum olfactorium, nucleus accumbens, hypothalamus, amygdala, striatum, pons and medulla. Tyrosine hydroxylase activity was measured by the radiometric method of Hendry & Iversen (1971). After 30 days of chronic treatment, the specific activity of tyrosine hydroxylase was decreased t o a value below the s...
متن کاملMolecular weight and structural nonequivalence of the mature alpha subunits of Torpedo californica acetylcholine receptor.
A discrepancy of about 20% exists between the molecular weight of the alpha subunit of Torpedo californica electroplax acetylcholine receptor as determined by gel electrophoresis of the mature protein (Mr 40,000 +/- 2000) and by nucleotide sequence analysis of cDNA (Mr approximately equal to 50,000). We demonstrate by amino acid sequence analysis that post-translational processing does not occu...
متن کاملThe Acetylcholine Receptor Channel from Torpedo
We recorded single channel currents activated by agonist binding to purified acetylcholine receptors from the electric organ of Torpedo californica reconstituted in planar lipid bilayers. Analysis of single channel records indicate that the acetylcholine receptor channel displays two kinetically distinct open states which differ in their mean open times but have similar channel conductances. Th...
متن کاملConsequences of alkaline treatment for the ultrastructure of the acetylcholine-receptor-rich membranes from Torpedo marmorata electric organ
After fixation with glutaraldehyde and impregnation with tannic acid, the membrane that underlies the nerve terminals in Torpedo marmorata electroplaque presents a typical asymmetric triple-layered structure with an unusual thickness; in addition, it is coated with electron-dense material on its inner, cytoplasmic face. Filamentous structures are frequently found attached to these "subsynaptic ...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1980
ISSN: 0014-5793
DOI: 10.1016/0014-5793(80)80616-8